LUPEOL AND LAURIC ACID ISOLATED FROM ETHYL ACETATE STEM EXTRACT OF Justicia secunda AND THEIR ANTIMICROBIAL ACTIVITY

Abstract

The method of cold maceration was used in the extraction of Justicia secunda starting with a non-polar (hexane) to a more polar solvent. The crude extracts of hexane, ethyl acetate, acetone, and methanol from the stem of were obtained using the polarity guided cold extraction method. Lupeol and Lauric acid are two well-known compounds that were discovered when a portion of the ethyl acetate extract of J. secunda was subjected to spectroscopic (1H NMR) structural elucidation. Based on the presence of Lupeol and Lauric acid in J. secunda stem, the plant could be a viable source of antimicrobial agents in the near future. The results of the antimicrobial activity obtained from the fractions of the Stem inhibited or exhibited activity against Methicillin resist Staph aureus, Vancomycin resistant enterococci, Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia, Helicobacter pylori, Campylobacter fetus, Proteus mirabilis, Pseudomonas aeruginosa, Candida tropicalis, and Candida krusei. The plant J. secunda traditionally employed in the treatment of anemic circumstance, blood boost, wound healing, abdominal pain and fertility issues. The overall results confirm the significance of the use of the plant in traditional medicinal treatment of anamic circumstances, blood boost, wound healing etc, in line with reported claims because of the presence of isolated compounds.