ANTIOXIDANT AND GLUCOSIDASE INHIBITION ACTIVITIES OF THE MUSHROOM: “PHAEOGYROPORUS PORTENTOSUS”

Abstract

The methanol, ethanol and water extracts of the mushroom, Phaeogyroporus portentosus, were evaluated for antioxidant activity using (2,2-diphenyl-1-picrylhydrazyl) DPPH, for free radical scavenging activity (RSA), total phenolic content (TPC) and ferric reducing antioxidant power (FRAP).  The methanol extract only of P. portentosus was also investigated for its ability to inhibit some commercially available glucosidase enzymes like alpha-D-glucosidases , alpha-D-galactosidase, alpha-D-mannosidase and beta-D-glucosidase. Results revealed that of all extracts tested for antioxidant activity, methanol extract possessed the highest RSA with an IC50 of 0.62±0.008 mg/mL and the highest concentration of TPC 2.73 mg GAE/g of extract. But water extract showed the highest FRAP value with IC50 of 755.87 ±23.83 mg/mL. The methanol extract also showed the greatest inhibitory activity against alpha-D-glucosidase enzyme isolated from Bacillus sterothermophillus (84.5%) more than all the other glucosidase enzymes tested. The results showed that methanol extract of P. portentosus may act as a natural antioxidant agent and alpha-glucosidase inhibitor (AGI).Keywords: Phaeogyroporus portentosus, alpha-glucosidase inhibitor, DPPH, FRAP, TPC